moclo

Terminal-based plasmid map viewer, sequence editor, and cloning/mutagenesis workbench in pure Python

Modular cloning simulation with the MoClo framework in Python

Open-source CDS design and pre-synthesis sequence review for plant gene constructs.

A modular high-throughput approach for advancing synthetic biology in the chloroplast of Chlamydomonas

The published YTK and MYT Golden Gate toolkits as GenBank files - a starter library for GG Assembler

PlasmidProbe is a reference-guided tool for identifying evidence of expected plasmid features in Oxford Nanopore sequencing reads. It maps reads against an expected plasmid sequence and evaluates read coverage, identity, depth, and support across annotated genetic features without requiring de novo plasmid assembly.

A Streamlit and command-line planner for Golden Gate and MoClo DNA assembly reactions.

A single-page, no-backend tool for planning Golden Gate and MoClo assemblies. Enter stock concentrations to get pipetting volumes and a recommended digestion–ligation thermocycler program, plus a searchable library of acceptors, parts, and linkers you can extend with your own (including GenBank import).

Design Golden Gate assemblies in the Yeast Toolkit (YTK) standard. Local desktop-in-browser app: detection by digest, never by annotation.