The two published Golden Gate toolkits for yeast, as GenBank files, ready to open in GG Assembler:
ggasm init ytk --from https://github.com/Thatguy027/ytk-starter-library.git
ggasm serve ytk192 records, flat, at the top level — 121 of them usable parts, covering every position of the YTK part circle, with no detection conflicts. Enough to design and simulate a complete cassette without adding anything of your own.
| What | Where it comes from | |
|---|---|---|
pYTK001–pYTK096 |
Yeast Toolkit | Lee, DeLoache, Cervantes & Dueber, ACS Synth. Biol. 2015. Addgene kit #1000000061 |
pMYT001–pMYT096 |
a YTK-compatible toolkit | Deposited at Addgene; each record carries its own Addgene ID in its COMMENT |
Nothing is organised into folders on purpose. What a plasmid is gets decided
by simulating the digest, never by its file name or its annotations, so a
folder called promoters/ would be a claim the app ignores and a human might
believe.
These are not our plasmids. They are two published kits, redistributed as sequence files so that the app has something to open on a machine that has nothing. Both were deposited at Addgene by the labs that made them, and the citation above is the one to use — not this repository.
A sequence file is also not a tube. Nothing here gets you the material: that means Addgene and a UBMTA, as it always did.
The underlying sequences are published scientific data and are not claimed by anyone here. We assert no copyright over them, and nothing in this repository is intended to add a restriction the depositing labs did not place on their own work.
What little is ours — the selection, the file layout, and any corrections to the records — is offered under CC BY 4.0, text in LICENSE. In practice: use these freely, and cite the papers above rather than this repository.
If you are one of the depositing authors and would rather these were not mirrored here, open an issue and they will come down.