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ab790e0
adding new galaxy-workflow
OEAdebayo 38bc962
Update workflows/epigenetics/correlation-of-samples-on-peaks/README.md
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workflows/epigenetics/correlation-of-samples-on-peaks/.dockstore.yml
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| version: 1.2 | ||
| workflows: | ||
| - name: correlation-of-samples-on-peaks | ||
| subclass: Galaxy | ||
| publish: true | ||
| primaryDescriptorPath: /correlation-of-samples-on-peaks.ga | ||
| testParameterFiles: | ||
| - /correlation-of-samples-on-peaks-tests.yml | ||
| authors: | ||
| - name: Lucille Delisle | ||
| orcid: 0000-0002-1964-4960 | ||
| - name: Alexandre Mayran | ||
| orcid: 0000-0002-1228-0308 | ||
| - name: Olusegun E. Adebayo | ||
| orcid: 0009-0003-9444-3944 |
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workflows/epigenetics/correlation-of-samples-on-peaks/CHANGELOG.md
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| # Changelog | ||
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| ## [0.1] 20206-07-06 | ||
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| First release. |
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workflows/epigenetics/correlation-of-samples-on-peaks/README.md
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| # correlation of samples on peaks: | ||
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| ## Input datasets | ||
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| - A collection (list) of peak files. | ||
| - A collection (list) of BAM or CRAM files | ||
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| ## Inputs values | ||
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| - Reference genome: this field will be adapted to the genomes for bedtools sort (dbkeys table) | ||
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| ## Processing | ||
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| - The workflow will concatenate the list of peak files given as input | ||
| - Sorts and merges the concatenated BED file by Chromosome using `bedtools sortBED` and `bedtools mergeBED`, respectively. | ||
| - Computes the read coverages for each BAM/CRAM file on the sorted and merged list of genomic region present in the bed files using `multiBamSummary`. | ||
| - Calculate and visualize pairwise correlation values between the read coverages using the tool `plotCorrelation`. | ||
| - The workflow produces three outputs: | ||
| - a compressed numpy array containing the raw count of the read coverages (i.e., the output of `multiBamSummary`) in .npz format | ||
| - The same raw count of the read coverages (i.e., the output of `multiBamSummary`) in .tabular format | ||
| - a plot of pairwise correlation values between the read coverages (i.e., the output of `plotCorrelation`). | ||
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...ows/epigenetics/correlation-of-samples-on-peaks/correlation-of-samples-on-peaks-tests.yml
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| - doc: Test outline for correlation-of-samples-on-peaks | ||
| job: | ||
| Collection of peak files: | ||
| class: Collection | ||
| collection_type: list | ||
| elements: | ||
| - class: File | ||
| identifier: peak2 | ||
| path: test-data/peak2.bed | ||
| - class: File | ||
| identifier: peak1 | ||
| path: test-data/peak1.bed | ||
| BAM or CRAM files: | ||
| class: Collection | ||
| collection_type: list | ||
| elements: | ||
| - class: File | ||
| identifier: sample2 | ||
| path: test-data/sample2.bam | ||
| - class: File | ||
| identifier: sample1 | ||
| path: test-data/sample1.bam | ||
| Reference genome: mm39 | ||
| outputs: | ||
| correlation plot: | ||
| path: test-data/correlation plot.png | ||
| deeptool raw counts: | ||
| path: test-data/deeptool raw counts.tabular | ||
| sort: true | ||
| deeptools coverage matrix: | ||
| path: test-data/deeptools coverage matrix.deeptools_coverage_matrix | ||
| compare: sim_size |
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