Single-cell RNA-seq analysis workflow (Seurat + Scanpy). QC → HVG → PCA → UMAP → Leiden → marker annotation. POC: 10x PBMC 3k — 2,643 cells post-QC, 7 Leiden clusters mapped to canonical T/B/Monocyte/NK lineages.
End-to-end spatial transcriptomics pipeline for 10x Genomics Visium human brain glioblastoma — cell type annotation, GBM subtype characterization, and spatial neighborhood analysis
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