final project submission for John Kalemkerian, Katherine Urasky, and Meagan Luck

bioinformaticsProject/computingproject.sh

@@ -0,0 +1,55 @@
+# Determine which isloated colonies of methanogens are the best candidates for growth experiments based on the presence of the McrA gene and multiple copies of the HSP70 gene.
+# Usage: bash computingproject.sh
+# Enter remote crc terminal
+# Be sure to have hmmsearch, hmmbuild, and muscle in the same working directory
+
+# Combine all hsp.fasta and mcrA.fasta into two separate files
+cat hsp*.fasta >> hspref.fasta
+cat mcrA*.fasta >> mcrAref.fasta
+
+# Use muscle to align reference sequences into two files, one for hsp70 and one for mcrA. This will generate .afasta files.
+./muscle -in hspref.fasta -out hspref.afasta
+./muscle -in mcrAref.fasta -out mcrAref.afasta
+
+# Input .afasta files into hmmbuild to generate two profile hmm's, one for hsp70 and one for mcrA.
+./hmmbuild hsphmm.txt hspref.afasta
+./hmmbuild mcrAhmm.txt mcrAref.afasta
+
+# Use hmmsearch to search for patterns in aligned sequences. Repeat for both hsp70 and mcrA. Match.txt files will contain result output tables.
+for file in proteome*.fasta
+do
+./hmmsearch --tblout hspmatch$file.txt hsphmm.txt $file
+done
+
+for file in proteome*.fasta
+do
+./hmmsearch --tblout mcrAmatch$file.txt mcrAhmm.txt $file
+done
+
+# Interpret results:
+
+# Generate a text file with the names of candidate pH-resistant methanogens based on tabular hmmsearch results.
+# hsp:
+for file in hspmatchproteome*.txt
+do
+echo $file | tr '\n' ' ' >> hspresults.txt
+grep "WP_" $file | wc -l >> hspresults.txt
+done
+
+grep -E "[23456789]$" hspresults.txt > results.txt
+
+# mrcA:
+for file in mcrAmatchproteome*.txt
+do
+echo $file | tr '\n' ' ' >> mcrAresults.txt
+grep "WP_" $file | wc -l >> mcrAresults.txt
+done
+
+grep -E "[123456789]$" mcrAresults.txt >> results.txt
+
+#Generate final answer
+cut -d _ -f 2 results.txt | cut -d . -f 1 | sort | uniq -c | sort | grep -E "2 " | tr -s ' ' | cut -d " " -f 3 > finalresults.txt
+
+# Rationale: The final results file lists the best 8 isolated candidate colonies because of the presence of the McrA gene to produce methane and the presence of two or greater HSP70 genes to confer pH resistance.  
+
+# Generate a summary table of best candidate results with proteome_number, number_of_mcrA_genes, number_of_HSP70_genes

bioinformaticsProject/computingproject1.sh

@@ -0,0 +1,54 @@
+
+# Determine which isloated colonies of methanogens are the best candidates for growth experiments based on the presence of the McrA gene and multipl$
+# Usage: bash computingproject.sh
+# Enter remote crc terminal
+# Be sure to have hmmsearch, hmmbuild, and muscle in the same working directory
+
+# Combine all hsp.fasta and mcrA.fasta into two separate files
+cat hsp*.fasta >> hspref.fasta
+cat mcrA*.fasta >> mcrAref.fasta
+
+# Use muscle to align reference sequences into two files, one for hsp70 and one for mcrA. This will generate .afasta files.
+./muscle -in hspref.fasta -out hspref.afasta
+./muscle -in mcrAref.fasta -out mcrAref.afasta
+
+# Input .afasta files into hmmbuild to generate two profile hmm's, one for hsp70 and one for mcrA.
+./hmmbuild hsphmm.txt hspref.afasta
+./hmmbuild mcrAhmm.txt mcrAref.afasta
+
+# Use hmmsearch to search for patterns in aligned sequences for both hsp70 and mcrA. Match.txt file will contain result output tables.
+for file in proteome*.fasta
+do
+./hmmsearch --tblout hspmatch$file.txt hsphmm.txt $file
+./hmmsearch --tblout mcrAmatch$file.txt mcrAhmm.txt $file 
+done
+
+
+# Interpret results:
+
+# Generate a text file with the names of candidate pH-resistant methanogens based on tabular hmmsearch results.
+# hsp:
+for file in hspmatchproteome*.txt
+do
+echo $file | tr '\n' ' ' >> hspresults.txt
+grep "WP_" $file | wc -l >> hspresults.txt
+done
+
+grep -E "[23456789]$" hspresults.txt > results.txt
+
+# mrcA:
+for file in mcrAmatchproteome*.txt
+do
+echo $file | tr '\n' ' ' >> mcrAresults.txt
+grep "WP_" $file | wc -l >> mcrAresults.txt
+done
+
+grep -E "[123456789]$" mcrAresults.txt >> results.txt
+
+#Generate final answer
+cut -d _ -f 2 results.txt | cut -d . -f 1 | sort | uniq -c | sort | grep -E "2 " | tr -s ' ' | cut -d " " -f 3 > finalresults.txt
+
+# Rationale: The final results file lists the best 8 isolated candidate colonies because of the presence of the McrA gene to produce methane and the$
+
+# Generate a summary table of best candidate results with proteome_number, number_of_mcrA_genes, number_of_HSP70_genes
+

bioinformaticsProject/hsp70gene_01.fasta

@@ -0,0 +1,2 @@
+>sp|Q9UXR0.1|DNAK_METTE RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MGKILGIDLGTTNSCMAVMEGGEAVVIPNAEGARTTPSVVGFSKKGEKLVGQVAKRQAISNPENTVYSIKRHMGEPNYKVTLQGKHNTPQEISAMILQKLKADAEAYLGEEIKQAVITVPAYFNDAQRQATKDAGTIAGLEVLRIINEPTAASLAYGLDKGEVEQTILVYDLGGGTFDVSILEIGGGVFEVKATSGDTHLGGDDFDQRIVNYLLAEFKKTEGIDLSKDRAVLQRLTDAAEKAKIELSGVASTNINLPFLTVGPDGEPKHLDIDLTRAQFQKMTEDLLEKTLVSMRQALSDAKLTPNDIDKVILIGGATRMPAVVELVENFTGKKPYKNINPDEAVAIGAAIQAGVLGGEVKDILLLDVTPLTLGIETLGGIATPLIPRNTTIPTRKSQIFSTAADNQPSVEIHVLQGERGIASENKTLGRFTLDGIPPAPRGVPQIEVTFDIDANGILHVSAKDLGTGREQSISIQKPGGLTDAEIERMIKDAELHAEEDRKRKEEVETRNNAETLINAAEKTLKEAGDVATEDQKSKVNAAIDDLKKALEGKDAEEIKAKTEALQEAIYPISTAMYQKAAQQAQQAAGGAAGRTDAKGPEETVVDAIMR

bioinformaticsProject/hsp70gene_02.fasta

@@ -0,0 +1,2 @@
+>sp|Q8TQR2.1|DNAK_METAC RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MAKILGIDLGTTNSCMAVMEGGDAVVLPNAEGSRTTPSVVGFSKKGEKLVGQVAKRQAISNPENTVYSIKRHMGEANYKVTLNGKTYTPQEISAMILQKLKTEAEAYLGETIKQAVITVPAYFNDAQRQATKDAGSIAGLEVLRIINEPTAASLAYGLDKGDVDQKILVYDLGGGTFDVSILELGGGVFEVKSTSGDTHLGGDDFDQRVIDHLLAEFKKTEGIDLSKDKAILQRLKDAAEKAKIELSGVTVTNINLPFLTVGPDGEPKHMDIDLTRAQFQKMTEDLLEKTLVSMRRALSDSKLTPNDLDKVILVGGATRMPAVVELVENFTGKKPYKNINPDEAVAIGAAIQAGVLGGEVKDVLLLDVTPLTLGIETLGGIATPLIQRNTTIPTKKSQIFSTAADNQPSVEIHVLQGERGIASENKTLGRFTLDGIPPAPRGIPQIEVAFDIDANGILHVNAKDLGTGKEQSISIQKPGGLSDAEIERMVKDAELHAEEDKKRKEEVETRNNAEALINAAEKTIKEAGDVATEDQKSKVNAAIEDLKKALEGKDTEEVKAKTEALQEAVYPISTAMYQKAQQEAQQASGEAGSADARGPDETVVDADYEVVDDEKRK

bioinformaticsProject/hsp70gene_03.fasta

@@ -0,0 +1,2 @@
+>sp|Q01100.2|DNAK_HALMA RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MASNKILGIDLGTTNSAFAVMEGGDPEIIVNGEGERTTPSVVAFDDGERLVGKPAKNQAVKNPDETIQSIKRHMGEDDYSVELDGEEYTPEQVSAMILQKIKHDAEEYLGDEIEKAVITVPAYFNDRQRQATKDAGEIAGFEVERIVNEPTAAAMAYGLDDESDQTVLVYDLGGGTFDVSILDLGGGVYEVVATNGDNDLGGDDWDHAIIDYLADEFEAEHGIDLRDDRQALQRLTEAAEEAKIELSSRKETRINLPFIATTDDGPLDLEQKITRAKFESLTEDLIERTVGPTEQALADADYTKSDIDEVILVGGSTRMPQVQDQVEEMTGQEPKKNVNPDEAVALGAAIQAGVLSGDVDDIVLLDVTPLSLGVEVKGGLFERLIDKNTTIPTEESKIFTTAQDNQTQVQIRVFQGEREIAEENELLGAFALSGIPPAPAGTPQIEVSFNIDENGIVNVEAEDKGSGNKEDITIEGGAGLSDDQIEEMQQEAEQHAEEDEQRRERIEARNEAEASVRRAETLLDENEEEIDEDLQSDIEAKIEDVEEVLEDEDATKEDYEAVTETLSEELQEIGKQMYQDQAQQAAGGAAGAGPGGAAGPGGAAGPGGAAGGAAEQGEEYVDADFEDVEESDEDE

bioinformaticsProject/hsp70gene_04.fasta

@@ -0,0 +1,2 @@
+>sp|P50023.2|DNAK_THEAC RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MSKIIGIDLGTSNSAAAVVISGKPTVIPSSEGVSIGGKAFPSYVAFTKDGQMLVGEPARRQALLNPEGTIFAAKRKMGTDYKFKVFDKEFTPQQISAFILQKIKKDAEAFLGEPVNEAVITVPAYFNDNQRQATKDAGTIAGFDVKRIINEPTAAALAYGVDKSGKSEKILVFDLGGGTLDVTIMDFGDGVFQVLSTSGDTRLGGTDMDEAIVNYIADDFQKKEGIDLRKDRSAYIRLRDAAEKAKIELSTTLSTDIDLPYITVTNSGPKHIKMTLTRAKLEELISPIVERVKGPIDKALEGAKLKKTEITKLLFVGGPTRIPYVRKYVEDYLGIKAEGGVDPMEAVAIGAAIQGAVLKGEIKDIVLLDVTPLTLSVETLGGIATPIIPANTTIPVRKSQIFTTAEDMQTTVTIHVVQGERPLAKDNVSLGMFNLTGIAPAPRGVPQIEVTFDIDSNGILNVTAVDKATGKKQGITITASTKLSKEEIERMKKEAEQYAEQDRKAKEQIELLNNAESLAYSVEKTLNDAGDKVDKETKERLTNEVKDLRKAIEEKNTENVKTLMDKLSKDIQEVGAKMYQQASANTQQSAQSNSQNSGSSDGKTVDAEYKEKS

bioinformaticsProject/hsp70gene_05.fasta

@@ -0,0 +1,2 @@
+>sp|Q97BG8.1|DNAK_THEVO RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MSKIIGIDLGTSNSAAAVVISGKPTVIPSSEGVSIGGKAFPSYVAFTKDGQMLVGEPARRQALLNPEGTIFAAKRKMGTDYKFKVFDKEFTPQQISAFILQKIKKDAEAFLGEPVNEAVITVPAYFNDNQRQATKDAGTIAGFDVKRIINEPTAAALAYGVDKSGKSEKILVFDLGGGTLDVTIIEISKRPNVQVLSTSGDTQLGGTDMDEAIVNYIADDFQKKEGIDLRKDRGAYIRLRDAAEKAKIELSTTLSSDIDLPYITVTSSGPKHIKMTLTRAKLEELISPIVERVKAPIDKALEGAKLKKTDITKLLFVGGPTRIPYVRKYVEDYLGIKAEGGVDPMEAVAIGAAIQGAVLKGEIKDIVLLDVTPLTLSVETLGGIATPIIPANTTIPVRKSQVFTTAEDMQTTVTIHVVQGERPLAKDNVSLGMFNLTGIAPAPRGIPQIEVTFDIDSNGILNVTAVDKATGKKQGITITASTKLSKDEIERMKKEAEQYAEQDRKMKEQIETLNNAESLAYSVEKTLNEAGDKVDKETKDRILSEVKDLRKAIEEKNMDNVKTLMEKISKDIQEVGTKMYQSASSTTQTGSGNQNSSKQENDKTVDAEYKEKS

bioinformaticsProject/hsp70gene_06.fasta

@@ -0,0 +1,2 @@
+>sp|O27351.1|DNAK_METTH RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MAKKEKILGIDLGTSNSAAAVLIGGKPTIIPSAEGASQYGKSFPSCVAFTEDGQMLVGEPARRQAVTNPENTITAIKRSMGTDRKVKVHGKEYTPQEISAFILQKIKKDAEAFLGEEIKKAVITVPAYFDDNQRTATKDAGTIAGLDVVRLVNEPTAASLAYGLDKEDEDMVIMVFDLGGGTLDVTIMEFGGGVFEVRSTSGDTQLGGTDMDNAIMNYLAEEFKMETGIDLMEDDQAVQRLREAAEKAKIELSTTLTTEVNLPYITVAQDGPKHLIKTITRAKLEELVDPIVQKCAGPMEQALRDAGMTREDVDKIILVGGPTRMPIVQKFVEDFIGKPVERGIDPMECVAMGAAIQGGVLAGEIKDLVLLDVTPLSLGIETLGGVFTKLIERNTTIPTRKSQIFSTAADNQTSVDIHVLQGERPMAADNTSLGRFQLVGIPPAPRGVPQIEVTFDIDANGILNVSAKDLGTGKEQAITITAPNKLSEEEIKQKIEEAKKHAEEDRRKQEEIEIRNNADSMIYTAEKTLDELGDKVPAEKKEEVEKQVRELRELIAGDDIQAIKSKTEELTKTVQEIGVSYIPAGSTATSSTAAGR

bioinformaticsProject/hsp70gene_07.fasta

@@ -0,0 +1,2 @@
+>sp|A9A135.1|DNAK_NITMS RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MGKVIGIDLGTSNSAAAVMMGGKPTIIPAAEGQTAAGKAFPSVVAFSKEGELLVGEPARRQAVTNPDNTIIAAKRKMGSDYTFKIQDKEYKPQQISSFILQKIKKDAEAFVGETVEKAVITVPAYFDDNQRQATKDAGTIAGLDVVRIINEPTAASLAFGLDKAKEDMKILVFDFGGGTLDVTIMEMGGGVFEVMSTSGDTQLGGTDMDKVLIDYIVDEFKKKEGVDLSQDTTAMTRIREAAEKAKIELSTVMETDVNLPFIAHDPSSGAKNLELRLTRSKLDELIGPIVDRCKPSIQKALEDAKLSNSDINKIVMIGGPTRIPLVKKFVSEVIGKEVESGVDPMEAVAMGAAIQAGIIAGDVTSDIVLLDVTPLTLGIETLGGVREPLIERNTTIPTSKGKVFTTAADNQTAVTIHVVQGERPMATDNVSLGSFNLTDLPPAPRGVPQIEVKFDIDANGIINVTAKDLGTQKEAKITIETKTKLSEEEIEKLKEDAEKFSEEDKKKKEKIDLKNEAESYIYTTEKLVNHDLKDKISQEQGIKITDAVKEVKEVLDKEPEELKPKLEALQSIVNEVTTELYKNAAPPPGADGQQGADGQQGADGQQGADGQQGADGQQGADGQTTESSSNDETKTN

bioinformaticsProject/hsp70gene_08.fasta

@@ -0,0 +1,2 @@
+>sp|Q6L0S7.1|DNAK_PICTO RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MSKIIGIDLGTSNSAAAVVISGKPTVIPAAEGVSLGGKAFPSYVAFTKDGQLLVGEPARRQALLNPEGTVYAAKRKMGTDYKYKIFGKEYTPQQISAFILQKIKRDAEAFLGEPVTDAVITVPAYFNDNQRQATKDAGAIAGLNVRRIINEPTAACLAYGIDKLNQTLKIVIYDLGGGTLDVTIMDFGQGVFQVLSTSGDTHLGGTDMDEAIVNFLADNFQRENGIDLRKDHSAYIRLRDAAEKAKIELSTVLETEINLPYITATQDGPKHLQYTLTRAKFEELIAPIVDRSKVPLDTALEGAKLKKGDIDKIILIGGPTRIPYVRKYVEDYFGRKAEGGVDPMEAVAMGAAIQGAVLAGEVKDIVLLDVTPLTLGIETLGGVMTPLIPANTTIPTKKSQIFTTAADMQTTVTIHVVQGERPLAKDDVSLGMFNLDGIPPAPRGVPQIEVTFDIDANGILNVSAKDLGTGKQQSISITATNKLSKDEIERMKKEAEQYAEQDKKAKEEIETINNAETLAYTAEKTINDAGDKIDESSKESVRSIVKDLRDAISSKDINKIKELSEKLTKEIQEIGTKMYQSQATQGTSQNSSQNNNSQNNNGDTVDADFKESK

bioinformaticsProject/hsp70gene_09.fasta

@@ -0,0 +1,2 @@
+>sp|A0RZ01.1|DNAK_CENSY RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MAKVIGIDLGTSNSAAAVVMGGKPTIIPAAEGATVGGKAFPSVVAFSKDGDLLVGEPARRQAVTNPENTIMAAKRKMGSEHIFKAGGKDYKPQQISSFILQKIKKDAEAFVGEPVGQAVITVPAYFDDNQRQATKDAGTIAGLDVVRIINEPTAASLAFGLDKSKQDMKILVFDFGGGTLDVTVMEMGGGVFEVMSTSGDTQLGGTDMDKAIIDYIVDDFRKREGVDLSQDSTALTRVREAAEKAKIELSTVMETDVNLPFISHDSSGPKNLELRLTRAKLEELVGPIIDRCRPSIEKALADAKVSPSDIAKIVMVGGPTRMPAVKKFVSSVTGKESESGVDPMEAVAMGAAIQAGIIAGDVTSDIVLLDVTPLTLGIDTLGGVREPLIERNTTIPTSKSKVFTTAVDNQTAVTIHVVQGERPMTSDNVSLGSFNLTDLPPAPRGIPQIEVKFDIDANGIINVTAKDLGTQKEAKITIESSSKLSKDEIEKLREDAEKFSDEDEKKKGKVELKNEAESFVYTAEKLINHDLKDKITQEQGIKISDGIREVKEALDQDPDILRPKLDSLKAMVNEITTEMYQQAAAQGAAGGAAGGAAGGAAGGAAGDAAGAAGDSTGDAAGAAGGPSEGPAGDAGAGESAGPEPGDDGQPKPGPAA

bioinformaticsProject/hsp70gene_10.fasta

@@ -0,0 +1,2 @@
+>sp|P0CW12.1|DNAK_METMZ RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MAKILGIDLGTTNSCVAVMEGGEAVVIPNAEGSRTTPSVVGFSKKGEKLVGQVAKRQAISNPDNTVYSIKRHMGEANYKVTLNGKDYTPQEISAMILQKLKADAEAYLGETIKQAVITVPAYFNDSQRQATKDAGAIAGLEVLRIINEPTAASLAYGLDKGDIDQKILVYDLGGGTFDVSILELGGGVFEVKSTSGDTHLGGDDFDQRVIDYLLAEFKKSEGIDLSKDKAVLQRLKDAAEKAKIELSGVANTNINLPFLTVGTDGEPKHMDIDLTRAQFQKMTEDLLEKTLVSMRRALSDAKLTPNDLDKVILVGGATRMPAVVELVENFTGKKPYKNINPDEAVAIGAAIQAGVLGGEVKDVLLLDVTPLTLGIETLGGIATPLIQRNTTIPTKKSQIFSTAADNQPSVEIHVLQGERGIASENKTLGRFILDGIPPAPRGIPQIEVTFDIDANGILHVSAKDLGTGKQQSISIQKPGGLSDDEIERMVKDAEMHAEEDRKRKEEVEIRNNAEALINAAEKTIKEAGDLATEDQKSKVNAAIEDLKKALEGKDAEDIKAKTEALQESVYPISTAMYQKAQQAQQAAGGEGGAAGTDARGPDETVVDADYEVVDDEKRK

bioinformaticsProject/hsp70gene_11.fasta

@@ -0,0 +1,2 @@
+>sp|P0CW13.1|DNAK_METMA RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MAKILGIDLGTTNSCVAVMEGGEAVVIPNAEGSRTTPSVVGFSKKGEKLVGQVAKRQAISNPDNTVYSIKRHMGEANYKVTLNGKDYTPQEISAMILQKLKADAEAYLGETIKQAVITVPAYFNDSQRQATKDAGAIAGLEVLRIINEPTAASLAYGLDKGDIDQKILVYDLGGGTFDVSILELGGGVFEVKSTSGDTHLGGDDFDQRVIDYLLAEFKKSEGIDLSKDKAVLQRLKDAAEKAKIELSGVANTNINLPFLTVGTDGEPKHMDIDLTRAQFQKMTEDLLEKTLVSMRRALSDAKLTPNDLDKVILVGGATRMPAVVELVENFTGKKPYKNINPDEAVAIGAAIQAGVLGGEVKDVLLLDVTPLTLGIETLGGIATPLIQRNTTIPTKKSQIFSTAADNQPSVEIHVLQGERGIASENKTLGRFILDGIPPAPRGIPQIEVTFDIDANGILHVSAKDLGTGKQQSISIQKPGGLSDDEIERMVKDAEMHAEEDRKRKEEVEIRNNAEALINAAEKTIKEAGDLATEDQKSKVNAAIEDLKKALEGKDAEDIKAKTEALQESVYPISTAMYQKAQQAQQAAGGEGGAAGTDARGPDETVVDADYEVVDDEKRK

bioinformaticsProject/hsp70gene_12.fasta

@@ -0,0 +1,2 @@
+>sp|A0B747.1|DNAK_METTP RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MSKIIGIDLGTSNSAAAVLIGGRPTIIPSAEGTSLGGKAFPSYVAFTKDGQVLVGEPARRQAITNPEGTITGIKRKMGTDYKVKVFGKEYTPEEISAHILRKIKQDAEAFLGEKVEKAVITVPAYFNDNQRQATKDAGTIAGLDVVRIINEPTAAALAYGLDKGGEQKIMVFDLGGGTLDVTIMEMGEGVFEVKSTSGDTQLGGRDMDERLLDYIVEKFRQETGINLRNDVMAMQRLREAAEVAKIELSSMLQTTINLPYITADASGPKHLNMTITRAKLEELIQDVLERCRGPMEQALSDAKLSKSDIDKIILVGGPTRMPAVQEFVKRVMGKDVERGVDPMECVAMGAAIQAGVLAGEVKDILLLDVTPLSLGVETLGGIMTRLIERNTTIPTRKSQIFTTAADNQTSVEIHVLQGERPLAKDNISLGRFTLVGIPPAPRGIPQIEVTFDIDANGILHVSAKDLATKKEQRITITAPHRLSKEEIEQKVKEAERYAQEDARRREEIETRNQADNLIYTTEKMLKEAGDVATSEQRERIEKAISELRDALSGKDVQEIKSKMEKLQNAVYELSAAMYQRAAQSQSGSATGSASGSTSGGKTVEADYEVVNDDKR

bioinformaticsProject/hsp70gene_13.fasta

@@ -0,0 +1,2 @@
+>sp|Q465Y6.1|DNAK_METBF RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MAKILGIDLGTTNSCMAVMEGGEAVVIPNAEGARTTPSVVGFSKKGEKLVGQVAKRQAISNPENTVYSIKRHMGDANYTVTLQGTQYKPQEISAMILQKLKTDAEAYLGETIKQAVITVPAYFNDAQRQATKDAGAIAGLDVLRIINEPTSASLAYGLDKGDIEQKILVYDLGGGTFDVSILELGGGVFEVKSTSGDTRLGGDDFDQRIVNYLLAEFRKIEGIDLSKDKAVLQRLTDAAEKAKIELSGVASTNINLPFLTVGADGEPKHLDIDLTRAQFQKMTEDLLEKTLVSMRQALSDAKLTPNDLDKVILVGGATRMPAVVELVENFTGKKPYKNINPDEAVAIGAAIQAGVLGGEVKDVLLLDVTPLTLGIETLGGIATPLIPRNTTIPTKKSQVFSTAADNQPSVEIHVLQGERGVASENKTLGRFTLDGIPPAPRGIPQIEVTFDIDANGILHVGAKDLGTGKEQSISIQKPGGLSDDEIDRMVKDAELHAEEDKKRKEDVETRNNAEALINAAEKTLKEAGDAATEDQKSKVTAAIDDLKKALEGKDSEDIKSKTEALQEAVYPISTAMYQKAQQQAQQAQQAEGEAGSHDAQGPDETVVDADYEVVDDEKRK

bioinformaticsProject/hsp70gene_14.fasta

@@ -0,0 +1,2 @@
+>sp|Q9HHB9.1|DNAK_HALMT RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MASNKILGIDLGTTNSAFAVMEGGDPEIIVNAEGDRTTPSVVAFTDDGERLVGKPAKNQAIQNPEHTIRSIKRHMGDDGYTVDIEGEEYTPEQISAMILQKIKRDAEDYLGDEVEKAVITVPAYFNDKQRQATKDAGEIAGLEVERIVNEPTAASMAYGLDDESNQTVLVYDLGGGTFDVSVLDLGGGVYEVVATNGDNELGGDDWDEAIIDWLATEFKNDHGIDLREDRQALQRLKDAAEEAKIELSSRKETTINLPFITATDSGPVHLEYDLTRAKFESLTKDLIDRTVEPTEQALEDAGYGKDDIDDVILVGGSTRMPQVLDKVEEILGSEPKKSVNPDEAVALGAAIQGGVLGGEVDDIVLLDVTPLSLGIEVKGGLFERLIEKNTTIPTEESKIFTTAADNQTTVQVRVFQGEREMAEDNELLGEFTLSGIPPAPAGTPQIEVGFNIDENGIVNVSAEDKGSGNAESITIEGGAGLSDDEIDRMQREAEQHAEEDKERRRAVEARNEAEGAVQRAETLLEENEENVDDELRADIEAAIEDVEAVLEDDDASTDELEDVTEDLSKELQEIGKRMYQQQAQAQAGGPGGAGGPGGADGPGADADPEDFVDADADFDDEDDDE

bioinformaticsProject/hsp70gene_15.fasta

@@ -0,0 +1,2 @@
+>sp|Q9HRY2.1|DNAK_HALSA RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MASEKILGVDLGTTNSAFAVMEGSDPEIITNEEGDRTTPSIVAHDDGELLVGKPAKNQAVQNPDQTIASIKRHMGEEDYTVALGDDEYTPEEISARILQKIKRDAEEYLGQDVEKAVITVPAYFNDRQRQATKDAGEIAGFDVERIVNEPTAASMAYGLDEDRDQTVLVYDLGGGTFDVSILDLGGGVYEVAATNGDNDLGGDDWDHAIIDHLADNFENEHGIDLREDRQALQRLTEAAEEAKIELSSRKETTVNLPFVTATDSGPVHLEQDITRATFESITEDLIERTVGPTEQALEDAGLSKSDIDDVILVGGSTRMPQVQAQVEDLVGQEPKKNVNPDEAVALGAAVQGGVLSGEVDDIVLVDVTPLSLGIEVKGGLFERLIEKNTAIPTTASKVFTTAADNQTSVQIRVFQGEREIASENELLGDFHLTGIPPAPAGTPQIEVTFEIDADGIVNVEAEDQGSGNAESITIEGGAGLSDEQIDEMQEDAEAHAEEDEQRRRRIEARNEAETAIQRAESLLEENEELVDEDLEADVNDAIDDVQAVLDEDEPEIDALETATEELSDTLQEIGKQAYQQQQDAQAGAAGGAGGMGGMGGMADGPGGAADADGDDEEYVDADFEDVDEE

bioinformaticsProject/hsp70gene_16.fasta

@@ -0,0 +1,2 @@
+>sp|Q18GZ4.1|DNAK_HALWD RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MASNKILGIDLGTTNSAFAVMEGDDPEIIVNAEGDRTTPSVVAMTDDEERLVGKPAKNQVIQNPDQTIRSIKRHMGEEDYTVELGGEDYTPEQVSAMILQKIKRDAEEYLGDEIEKAVITVPAYFNDRQRQATKDAGEIAGFEVDRIVNEPTAASMAYGLDDESNQTILVYDLGGGTFDVSVLDLGGGVYEVVATNGDNDLGGDDWDDAVIDWLAGEFEDNHGIDLRDDRQALQRLKDAAEEAKIELSSRKETTINLPFITATDSGPVHLEETLSRAKFESLTEDLIERTVGPTEQALEDAGYDDSDIDEVILVGGSTRMPQVREKVEDLLGTEPKKNVNPDEAVALGAAIQGGVLAGDVDDIVLLDVTPLSLGIEVKGGLFERLIDKNTTIPTEESKVFTTAAANQTSVNVRVFQGEREIAEENELLGEFQLAGIPPAPAGTPQIEVTFNIDENGIVNVEAEDQGSGNAESITIEGGAGLSDEEIEQMQEEAEAHAEEDERRRERIEARNSAESAVQRAETLLEENEEDIDDDLKESIEDEVESVEAVLEDEDATKEEIEDVTESLSSELQEIGKQMYDAQQAAAGAGAGAAGAGAGAGPGGMGDMGDMGDMGGAAGSGDADNEYVDADFEDVDDDTKDE

bioinformaticsProject/hsp70gene_17.fasta

@@ -0,0 +1,2 @@
+>sp|Q12WE6.1|DNAK_METBU RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MGKILGIDLGTTNSCMAVIEGGKPTVIPNAEGGRTTPSVVGFSKKGDKLVGQVAKRQMIANPGNSVSSIKRHIGEGDYKVNLSGKDYTPQEVSAMILRKLKDDAEAYLGETITQTVITVPAYFNDSQRQATKDAGQIAGLEVLRIINEPTAASLAYGLDKEEGDHKILVYDLGGGTFDVSILELGDGVFEVLSTSGNTHLGGDDFDQRITEFLVEEFKKAEGIDLSNDKAALQRLNDAAEKAKIELSGVASTNVNLPFITADSNGQPKHIDIDITRAQFEKMTEDLVAKTLESMKMALSDAKLTTKDIDRVLLIGGSTRTPAVYNLVKNFIGKDPYKNINPDEAVAVGAAIQAGVLSGEVHDVLLLDVTPLTMGIETLGGVATPLIERNTTIPVKKSQIFSTAADSQPSVEIHILQGERGIASANKTLGRFVLDGIPPAPRGLPQIEVTFDIDSNGILHVNAKDLGTGKEQSISIQKPGGLSDEEIERMVKDAELHAEEDKARKEEVETRNNADSLVNAAENTLKEAGDVATNEQKEQIEAAIADLKTALEGEDLEAIKSKTEALQESVYKVSAAMYEKAQKEASAGAEASEDASGPSSTGSASDDDVVDADYEVVDEDK

bioinformaticsProject/hsp70gene_18.fasta

@@ -0,0 +1,2 @@
+>sp|Q3IUI0.1|DNAK_NATPD RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MASNKILGIDLGTTNSAFAVMEGGDPEIIVNSEGERTTPSVVAFTDDGERLVGKPAKNQAVQNPEDTIQSIKRHMGEDDYTVEVGDDEYTPEQISAMILQKIKRDAEEYLGDDIEKAVITVPAYFNDRQRQATKDAGEIAGFEVERIVNEPTAAAMAYGLDDESDQTVLVYDLGGGTFDVSILDLGGGVYEVVATNGDNDLGGDDWDEAIIDYLADSFEEEHGIDLREDRQALQRLHEAAEEAKIELSSRKETNINLPFIAATDEGPLNLEESISRAKFESLTSDLVERTVGPTEQALDDAGYSKGDIDEVILVGGSTRMPMVQEKVEELTGQEPKKNVNPDEAVGLGAAIQGGVLSGDVDDIVLLDVTPLSLGIEVKGGLFERLIDKNTTIPTEASKVFTTAADNQTSVNIRVFQGEREIAEENELLGAFQLTGIPPAPAGTPQIEVTFNIDENGIVNVEAEDQGSGNKEDITIEGGVGLSDEEIEEMQEEAEKHAEEDEKRRERIEARNEAESTLQRAETLLDENEDAVDDDLRADIEASMDDLREVVEDEDADTDELTEATEALAEALQEIGKQMYQQQAGEGGAGAGAGAAGGMGGAGPGGMGGAGPGGMGGAGPGGMGGAGPGAGAGQQGDGEEFVDADFEDVDDEDDEDE

bioinformaticsProject/hsp70gene_19.fasta

@@ -0,0 +1,2 @@
+>sp|B9LUC7.1|DNAK_HALLT RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MASNKILGIDLGTTNSAFAVMEGDEPEIIANAEGDRTTPSVVAFADDGERLVGKPAKNQAVQNPDRTIQSIKRHMGEDGYTVEIGDEEYTPEQVSAMILQKIKRDAEEYLGDDVEKAVITVPAYFNDKQRQATKDAGEIAGFEVERIVNEPTAASMAYGLDDESDQTVLVYDLGGGTFDVSVLDLGGGVYEVVATNGDNDLGGDDWDEALIDHLAKEFKNNHGIDLREDRQALQRLKDAAEEAKIELSSKKETTVNLPFITATDSGPVHLEQSITRATFENLTSDLIERTVNPTEQALSDADYSKSDIDEVILVGGSTRMPQVQEQVEALVGQEPKKNVNPDEAVALGAAVQGGVLSGDVDDIVLLDVTPLSLGIEVKGGLFERLIEKNTTIPTEESKVFTTAADNQTSVNVRVFQGEREIAEENELLGAFQLSGIPPAPAGTPQIEVSFNIDENGIVNVEAEDQGSGNAESITIEGGVGLSDEEIDQMQEEAEKHKEEDEARRERIEARNEAETSIQRAETLLEENEEELDDDELVADIEAAIEDVEEVLEDEDADTDEIESATEALTEELQEIGKQMYEGQAAQAGPGGAGGAAGAGPGGMGGMGGAAGPGGAGGAGPGGADADDEEYVDADFEDVDDEDDA

bioinformaticsProject/hsp70gene_20.fasta

@@ -0,0 +1,2 @@
+>sp|B0R3H4.1|DNAK_HALS3 RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MASEKILGVDLGTTNSAFAVMEGSDPEIITNEEGDRTTPSIVAHDDGELLVGKPAKNQAVQNPDQTIASIKRHMGEEDYTVALGDDEYTPEEISARILQKIKRDAEEYLGQDVEKAVITVPAYFNDRQRQATKDAGEIAGFDVERIVNEPTAASMAYGLDEDRDQTVLVYDLGGGTFDVSILDLGGGVYEVAATNGDNDLGGDDWDHAIIDHLADNFENEHGIDLREDRQALQRLTEAAEEAKIELSSRKETTVNLPFVTATDSGPVHLEQDITRATFESITEDLIERTVGPTEQALEDAGLSKSDIDDVILVGGSTRMPQVQAQVEDLVGQEPKKNVNPDEAVALGAAVQGGVLSGEVDDIVLVDVTPLSLGIEVKGGLFERLIEKNTAIPTTASKVFTTAADNQTSVQIRVFQGEREIASENELLGDFHLTGIPPAPAGTPQIEVTFEIDADGIVNVEAEDQGSGNAESITIEGGAGLSDEQIDEMQEDAEAHAEEDEQRRRRIEARNEAETAIQRAESLLEENEELVDEDLEADVNDAIDDVQAVLDEDEPEIDALETATEELSDTLQEIGKQAYQQQQDAQAGAAGGAGGMGGMGGMADGPGGAADADGDDEEYVDADFEDVDEE

bioinformaticsProject/hsp70gene_21.fasta

@@ -0,0 +1,2 @@
+>sp|Q9UXR0.1|DNAK_METTE RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MGKILGIDLGTTNSCMAVMEGGEAVVIPNAEGARTTPSVVGFSKKGEKLVGQVAKRQAISNPENTVYSIKRHMGEPNYKVTLQGKHNTPQEISAMILQKLKADAEAYLGEEIKQAVITVPAYFNDAQRQATKDAGTIAGLEVLRIINEPTAASLAYGLDKGEVEQTILVYDLGGGTFDVSILEIGGGVFEVKATSGDTHLGGDDFDQRIVNYLLAEFKKTEGIDLSKDRAVLQRLTDAAEKAKIELSGVASTNINLPFLTVGPDGEPKHLDIDLTRAQFQKMTEDLLEKTLVSMRQALSDAKLTPNDIDKVILIGGATRMPAVVELVENFTGKKPYKNINPDEAVAIGAAIQAGVLGGEVKDILLLDVTPLTLGIETLGGIATPLIPRNTTIPTRKSQIFSTAADNQPSVEIHVLQGERGIASENKTLGRFTLDGIPPAPRGVPQIEVTFDIDANGILHVSAKDLGTGREQSISIQKPGGLTDAEIERMIKDAELHAEEDRKRKEEVETRNNAETLINAAEKTLKEAGDVATEDQKSKVNAAIDDLKKALEGKDAEEIKAKTEALQEAIYPISTAMYQKAAQQAQQAAGGAAGRTDAKGPEETVVDAIMR

bioinformaticsProject/hsp70gene_22.fasta

@@ -0,0 +1,2 @@
+>sp|Q0W874.1|DNAK_METAR RecName: Full=Chaperone protein DnaK; AltName: Full=HSP
+MSKILGIDLGTSNSEAAIMEGGKPVIIPSAEGARMFPSVVAFTKTGERLVGEAARRQAVTNPERTIIAIKRKMGTDYKVEIDGKKYTPQEISAMILQKIKQDAEAYIGEKITKAVITVPAYFNDNQRQATKDAGTIAGLEVLRVINEPTAAALAFGLDKKGSQKILVFDLGGGTLDVTIMEMGDGVFEVLSTSGDTQLGGTDMDNKIIDYIASQFKKDTGIDLRNDKMAMQRLRDAAEKAKIELSSTLQTQVNLPFITADASGPKHLDMMLTRATLEELVREVVERCYAPMRQAISDAKLTANQIDRIILVGGPTRMPIIIETIKKFFGKEPERGIDPMECVAMGASIQGGVLAGEVKDLLLLDVTPLSLGIETLGNVATRLIERNTTIPTRKSQVFSTAADNQTSVEIHVIQGERPMAYDNTTLGRFHLIGIPPAPRGIPQIEVTFDIDANGILNVKAKDLGTGKEQAITITASTKLDKSDIERMVKDAEKFAAEDAAKKEKAEVMNQADTLLYSAEKTLADAGDKITADQKERVTKGSDALREAQKTGDIEKIKAATADLTKTMNEVATVLYQAAQQQYQQQQAAEQAAQQQSGGQQASGSNPGKDPNVVDADYEVVNDKK