Custom barcodes not found

[termithorbor]

Hi,

I have designed a custom dual indexing approach based on the ONT Native barcoding primers:

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Ont_flank1 forward	Barcode forward	Ont_flank2 forward
AAGGTTAA	CACAAAGACACCGACAACTTTCTT	CAGCACCT
AAGGTTAA	ACAGACGACTACAAACGGAATCGA	CAGCACCT
AAGGTTAA	CCTGGTAACTGGGACACAAGACTC	CAGCACCT

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Ont_flank1 reverse	Barcode reverse	Ont_flank2 reverse
GGTGCTG	AAGAAAGTTGTCGGTGTCTTTGTG	TTAACCTTAGCAAT
GGTGCTG	TCGATTCCGTTTGTAGTCGTCTGT	TTAACCTTAGCAAT
GGTGCTG	GAGTCTTGTGTCCCAGTTACCAGG	TTAACCTTAGCAAT

My actual barcodes are surrounded by the ONT flanks.
I basically combine a forward and a reverse primer resulting in a unique primer-pair.
Is there a way to achive demultiplexing with porechop?

Thanks in advance.

[Dafnaa]

Hii, I'm trying the same thing, have you found a solution yet?