You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
{{ message }}
Repository navigation
Investigate pysam pipe close error during bounded remote BAM extraction #106
During Isovar's catalogue-wide SV RNA audit, bounded remote BAM extraction emits an unraisable pysam close error while handling a record-limit exception:
osteosarc.errors.RecordLimitError: Acquisition exceeds record limit
During handling of the above exception, another exception occurred:
OSError: [Errno 29] Closing failed: Illegal seek
Exception ignored in: 'pysam.libcalignmentfile.AlignmentFile.__dealloc__'
File "osteosarc/reads.py", line 251, in _run_bounded
with pysam.AlignmentFile(process.stdout, "rb") as bam:
OSError: [Errno 29] Closing failed: Illegal seek
Observed with released osteosarc 0.15.1, Python 3.12 on macOS, indexed remote RNA BAMs, max_records=500000, and nested batch retries after a record cap. The record-cap handling continues, but the close diagnostic is noisy and its interaction with timer-driven process termination should be checked. A separate leaf acquisition returned CalledProcessError after a truncated BGZF read; the current Isovar receipt records the error class/command but does not preserve the subprocess stderr, so these observations should not be assumed to have the same cause.
Public source in the affected pilot: https://sid-sijbrandij-osteosarc-dataset.s3.us-west-2.amazonaws.com/kamil/tumor/output/T2/outs/per_sample_outs/T2/sample_alignments.bam
Related implementation and reproducible frozen region plan: openvax/isovar#433 (batch e11a46eac1e8c5e207fcf974, recursively partitioned). Add a pipe/timeout/early-record-cap regression that verifies the primary exception survives cleanup and no partial BAM is published.
Activity