dual-crispr-process-nf

Process dual-sgRNA CRISPR functional genetic screening data

Installation

Nextflow

Install nextflow (DSL2) following the instructions at https://www.nextflow.io/docs/latest/getstarted.html

Apptainer

Install apptainer following the instructions at https://apptainer.org/docs/user/latest/quick_start.html#installation

dual-crispr-process-nf pipeline

The most convenient way is to install dual-crispr-process-nf is to use nextflow's built-in pull command

nextflow pull zuberlab/dual-crispr-process-nf

Test

Before you start, make sure nextflow and apptainer are properly installed on your system.

Clone git repository from Github and run the pipeline using the provided test data.

git clone https://github.com/ZuberLab/dual-crispr-process-nf.git
cd dual-crispr-process-nf
./test

Documentation

nextflow run zuberlab/dual-crispr-process-nf --help