diff --git a/Cargo.lock b/Cargo.lock
index 8519057..8aab4a8 100644
--- a/Cargo.lock
+++ b/Cargo.lock
@@ -67,12 +67,6 @@ dependencies = [
  "windows-sys",
 ]
 
-[[package]]
-name = "anyhow"
-version = "1.0.103"
-source = "registry+https://github.com/rust-lang/crates.io-index"
-checksum = "2a4385e2e34eb35d6b3efe798b9eb88096925d87726c0798709bf56d9ed84af3"
-
 [[package]]
 name = "approx"
 version = "0.5.1"
@@ -90,11 +84,10 @@ checksum = "f2032f911046de80f0a198e0901378627c33f59ea0ac00e363d481118bd70a53"
 
 [[package]]
 name = "bio"
-version = "3.0.0"
+version = "4.0.1"
 source = "registry+https://github.com/rust-lang/crates.io-index"
-checksum = "2169c40f17068bcf0563e5060ed6ccab694f68068133055f6e96bcdf987b3f32"
+checksum = "7ec70754e9fc444b545c0f4e3ac5547885dd83f297d642be4a260672aa59610b"
 dependencies = [
- "anyhow",
  "approx",
  "bio-types",
  "bit-set",
@@ -142,18 +135,21 @@ dependencies = [
 
 [[package]]
 name = "bit-set"
-version = "0.8.0"
+version = "0.10.0"
 source = "registry+https://github.com/rust-lang/crates.io-index"
-checksum = "08807e080ed7f9d5433fa9b275196cfc35414f66a0c79d864dc51a0d825231a3"
+checksum = "09ec2f926cc3060f09db9ebc5b52823d85268d24bb917e472c0c4bea35780a7d"
 dependencies = [
  "bit-vec",
 ]
 
 [[package]]
 name = "bit-vec"
-version = "0.8.0"
+version = "0.9.1"
 source = "registry+https://github.com/rust-lang/crates.io-index"
-checksum = "5e764a1d40d510daf35e07be9eb06e75770908c27d411ee6c92109c9840eaaf7"
+checksum = "b71798fca2c1fe1086445a7258a4bc81e6e49dcd24c8d0dd9a1e57395b603f51"
+dependencies = [
+ "serde",
+]
 
 [[package]]
 name = "buffer-redux"
diff --git a/Cargo.toml b/Cargo.toml
index d4b1893..b0760ae 100644
--- a/Cargo.toml
+++ b/Cargo.toml
@@ -9,7 +9,7 @@ description = "A tool to filter fastq files"
 # See more keys and their definitions at https://doc.rust-lang.org/cargo/reference/manifest.html
 
 [dependencies]
-bio = "3.0.0"
+bio = "4.0.1"
 clap = { version = "4.6.1", features = ["derive"] }
 crossbeam-channel = "0.5"
 rayon = "1.12.0"