Exclude some barcodes combinations ?

[alimatai]

Hello,

While analyzing sequencing data biologists sent me, I found quite a big issue with the F/R barcodes combinations.

For a fastq file, they provide two barcodes fasta file (F and R), both with 7 barcodes. The problem is that flexbar (used with the -b and -b2 parameters) seems to search for all possible F/R barcodes combinations (thus 49), while they used less combinations (19 in total).

My question is : is it possible to exclude barcodes combinations (or to specify which one to use) ?

Thanks.