From dab8f9244c015bd1a5698b5eb0ee3212e3eabcbf Mon Sep 17 00:00:00 2001
From: mite-bot <zdoucmm@gmail.com>
Date: Wed, 15 Apr 2026 11:30:26 +0200
Subject: [PATCH 1/2] Add submission UUID file

---
 .../5af77cd4-38a9-11f1-8f25-9b0d34c97d5b.json | 44 +++++++++++++++++++
 1 file changed, 44 insertions(+)
 create mode 100644 mite_data/data/5af77cd4-38a9-11f1-8f25-9b0d34c97d5b.json

diff --git a/mite_data/data/5af77cd4-38a9-11f1-8f25-9b0d34c97d5b.json b/mite_data/data/5af77cd4-38a9-11f1-8f25-9b0d34c97d5b.json
new file mode 100644
index 00000000..65db41c1
--- /dev/null
+++ b/mite_data/data/5af77cd4-38a9-11f1-8f25-9b0d34c97d5b.json
@@ -0,0 +1,44 @@
+{
+    "accession": "MITE9999999",
+    "status": "pending",
+    "changelog": [
+        {
+            "version": "1",
+            "date": "2026-04-15",
+            "contributors": [
+                "0000-0003-2971-646X"
+            ],
+            "reviewers": [
+                "BBBBBBBBBBBBBBBBBBBBBBBB"
+            ],
+            "comment": "New entry."
+        }
+    ],
+    "enzyme": {
+        "name": "RSAD2",
+        "databaseIds": {
+            "uniprot": "Q8WXG1",
+            "genpept": ""
+        },
+        "references": [
+            "doi:"
+        ]
+    },
+    "reactions": [
+        {
+            "evidence": {
+                "evidenceCode": [],
+                "references": [
+                    "doi:"
+                ]
+            },
+            "reactions": [
+                {
+                    "products": [
+                        ""
+                    ]
+                }
+            ]
+        }
+    ]
+}
\ No newline at end of file

From 430eafe79d6ea5702c20a474a24f802547b72234 Mon Sep 17 00:00:00 2001
From: mite-bot <zdoucmm@gmail.com>
Date: Wed, 15 Apr 2026 11:50:37 +0200
Subject: [PATCH 2/2] Update submission

---
 .../5af77cd4-38a9-11f1-8f25-9b0d34c97d5b.json | 49 +++++++++++++------
 1 file changed, 35 insertions(+), 14 deletions(-)

diff --git a/mite_data/data/5af77cd4-38a9-11f1-8f25-9b0d34c97d5b.json b/mite_data/data/5af77cd4-38a9-11f1-8f25-9b0d34c97d5b.json
index 65db41c1..31701890 100644
--- a/mite_data/data/5af77cd4-38a9-11f1-8f25-9b0d34c97d5b.json
+++ b/mite_data/data/5af77cd4-38a9-11f1-8f25-9b0d34c97d5b.json
@@ -1,6 +1,7 @@
 {
     "accession": "MITE9999999",
     "status": "pending",
+    "comment": "Radical SAM catalyzes formal dehydration of CTP to afford anti-viral ddhCTP",
     "changelog": [
         {
             "version": "1",
@@ -16,29 +17,49 @@
     ],
     "enzyme": {
         "name": "RSAD2",
+        "description": "Radical S-adenosyl methionine",
+        "references": [
+            "doi:10.1038/s41586-018-0238-4"
+        ],
         "databaseIds": {
-            "uniprot": "Q8WXG1",
-            "genpept": ""
+            "uniprot": "O70600",
+            "genpept": "CAA68971.1"
         },
-        "references": [
-            "doi:"
-        ]
+        "cofactors": {
+            "organic": [
+                "SAM"
+            ],
+            "inorganic": [
+                "FeS"
+            ]
+        }
     },
     "reactions": [
         {
-            "evidence": {
-                "evidenceCode": [],
-                "references": [
-                    "doi:"
-                ]
-            },
+            "tailoring": [
+                "Dehydration"
+            ],
+            "description": "Formal dehydration of CTP to afford anti-viral ddhCTP",
+            "reactionSMARTS": "[#6:1]1:[#6:7](-[#7:8]):[#7:6]:[#6:4](=[#8:5]):[#7:3](/[#6@@:9]2-[#8:13]-[#6@:12](/[#6:14]-[#8:15]-[#15@@:16](-[#8:19]-[#15@@:20](-[#8:23]-[#15:24](-[#8:27])(-[#8:26])=[#8:25])(\\[#8:22])=[#8:21])(\\[#8:18])=[#8:17])-[#6@@:11](\\[#8])-[#6@:10]-2\\[#8:29]):[#6:2]:1>>[#6:1]1:[#6:7](-[#7:8]):[#7:6]:[#6:4](=[#8:5]):[#7:3](/[#6@@:9]2-[#8:13]-[#6:12](-[#6:14]-[#8:15]-[#15@@:16](-[#8:19]-[#15@@:20](-[#8:23]-[#15:24](-[#8:27])(-[#8:26])=[#8:25])(\\[#8:22])=[#8:21])(\\[#8:18])=[#8:17])=[#6:11]-[#6@:10]-2\\[#8:29]):[#6:2]:1",
             "reactions": [
                 {
+                    "substrate": "Nc1ccn(C2OC(COP(=O)(O)OP(=O)(O)OP(=O)(O)O)C(O)C2O)c(=O)n1",
                     "products": [
-                        ""
-                    ]
+                        "Nc1ccn(C2OC(COP(=O)(O)OP(=O)(O)OP(=O)(O)O)=CC2O)c(=O)n1"
+                    ],
+                    "isIntermediate": true
                 }
-            ]
+            ],
+            "evidence": {
+                "evidenceCode": [
+                    "Heterologous expression",
+                    "Inference from genomic data and chemical structure",
+                    "In vitro assay"
+                ],
+                "references": [
+                    "doi:10.1038/s41586-018-0238-4"
+                ]
+            }
         }
     ]
 }
\ No newline at end of file