diff --git a/mite_data/data/MITE0000272.json b/mite_data/data/MITE0000272.json new file mode 100644 index 00000000..3309d5d8 --- /dev/null +++ b/mite_data/data/MITE0000272.json @@ -0,0 +1,68 @@ +{ + "accession": "MITE0000272", + "status": "active", + "comment": " Luc2 might be involved in C-20 hydroxylation", + "changelog": [ + { + "version": "1", + "date": "2026-03-26", + "contributors": [ + "0000-0002-6077-7490" + ], + "reviewers": [ + "0000-0002-9747-3423" + ], + "comment": "New entry." + } + ], + "enzyme": { + "name": "Luc2", + "description": "Cytochrome P450", + "references": [ + "doi:10.1038/s41429-022-00529-3" + ], + "auxiliaryEnzymes": [ + { + "name": "Luc6", + "description": "\u03b1/\u03b2 hydrolase", + "databaseIds": { + "uniprot": "A0A6J4B4M6", + "genpept": "BBQ09586.1" + } + } + ], + "databaseIds": { + "uniprot": "A0A6J4BC30", + "genpept": "BBQ09591.1" + } + }, + "reactions": [ + { + "tailoring": [ + "Monooxygenation", + "Other" + ], + "description": " Luc2 oxidizes the C-20, but Luc3 is required for the full oxidation of the C-20 methyl group into the carboxylic acid", + "reactionSMARTS": "[#7:1](-[#6:21](-[#6:24]-[#8:26])-[#6:22]-[#6:23]-[#8:25])-[#6:2](=[#8:7])-[#6:3]-[#6:4](=[#8:8])-[#6:5](-[#6:9])=[#6:6]-[#6:10]=[#6:11]-[#6:12]=[#6:13]-[#6:14]=[#6:15](-[#6:20])-[#6:16]=[#6:17](-[#6:19])-[#6:18]>>[#7]1-[#6:24](-[#6]2-[#6:3](-[#8]-2)(-[#6:4](=[#8:8])-[#6:5](-[#6:9])=[#6:6]-[#6:10]=[#6:11]-[#6:12]=[#6:13]-[#6:14]=[#6:15](-[#6:20])-[#6:16]=[#6:17](-[#6]-[#8])-[#6:19])-[#6:2]-1=[#8:7])(-[#8])-[#6:25]-[#6]-[#8]", + "reactions": [ + { + "substrate": "CC(C)=C/C(C)=C/C=C/C=C/C=C(\\C)C(=O)CC(=O)NC(CO)CCO", + "products": [ + "CC(=CC=CC=CC=C(C)C(=O)C12OC1C(O)(CCO)NC2=O)C=C(C)CO" + ], + "isIntermediate": false, + "description": "oxidation-cyclisation sequence with invovlement of Luc2 and Luc6" + } + ], + "evidence": { + "evidenceCode": [ + "Knock-out studies" + ], + "references": [ + "doi:10.1038/s41429-022-00529-3", + "doi:10.1016/j.chembiol.2013.07.004" + ] + } + } + ] +}