Use find-aa-substitutions.pl to identify amino acid substitutions.
Requirements:
- SNP files
- GFF3 file
- Genomic FASTA reference file
Use run-provean.pl to determine the putative functional severity of the amino acid changes.
Requirements:
- Amino acid substitution files
- CDS FASTA reference file
- PROVEAN installation
Use filter-provean-results.pl to filter PROVEAN results for a single gene, a file containing a list of genes, or all genes within a genomic range.
Version 0.4.0