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0,4 0,2 0,2 0,1 5,0 6,0 1,4 1,1 3,1 0,5 2,1 3,0 1,2 NA 4,1 NA 1,0 2,4 2,2 NA 1,2 0,1 2,0 2,3 4,5 NA 1,3 0,4 NA 4,0 6,0 NA 0,3 3,2 2,0 NA 0,3 +Contig16622 62 DeletedStuff GT:AD:DP:GQ:PL 2,0 1,0 2,0 3,0 1,0 2,0 2,0 1,0 NA 3,1 6,0 2,0 2,0 NA 1,0 4,0 3,0 2,0 NA 4,0 3,0 2,0 2,0 1,0 3,0 1,0 NA 3,0 4,0 4,0 1,0 2,0 4,0 1,0 2,0 1,0 6,0 2,0 3,0 1,0 2,0 3,0 7,0 1,0 NA 3,0 NA 4,0 2,0 3,0 1,0 3,0 1,0 6,0 6,0 3,0 3,0 1,0 3,0 2,0 NA 1,0 4,0 NA NA 1,0 3,0 4,0 6,0 NA 3,0 2,0 2,0 1,0 1,0 1,0 1,0 3,0 3,0 1,0 3,0 NA 4,0 4,0 NA 4,0 +Contig16634 73 DeletedStuff GT:AD:DP:GQ:PL 1,0 3,0 2,0 2,0 1,0 4,0 4,0 2,0 NA 3,0 3,0 3,0 1,0 3,0 3,0 3,0 2,0 4,0 2,0 2,0 2,0 2,0 2,0 2,0 2,0 NA 3,0 NA NA 4,0 1,0 1,0 3,0 4,0 2,0 2,0 3,0 1,0 2,0 3,0 2,0 1,0 6,0 1,0 3,0 3,0 6,0 7,0 2,0 3,0 2,0 1,0 1,0 3,0 4,0 2,0 3,0 2,0 3,0 6,0 3,0 1,0 7,0 1,0 7,0 8,0 1,0 3,0 3,0 3,0 4,0 4,0 6,0 2,0 1,0 2,0 6,0 3,0 1,0 3,0 3,2 3,0 4,0 1,0 2,0 1,0 +Contig16663 2 DeletedStuff GT:AD:DP:GQ:PL 1,0 NA NA NA NA 2,0 NA 5,0 NA 1,0 3,0 1,0 1,0 1,0 1,0 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@@ +#Ex 8 + +#Open files to read and write + +import re +vcffile = open("Cflorida.vcf","r") +outfile = open("CfloridaCounts.txt","w") + +#assign regex to variable names + +TXnames=r"[Cc][Ff](07)?\.[Aa](2)?" +FLnames=r"[Cc][Ff]\.[Gg][Aa2](Ii)?" +ACR=r"[01.]/[01.]:([0-9,.]+):[0-9.]+:[0-9.]+:[0-9,.]+" + +#loop over file +for Line in vcffile: + #strip end of line + Line = Line.strip() + + #ID header line + if "##" in Line: + #write unchanged header line to file + outfile.write(Line + "\n") + #ID second line + elif "#" in Line: + #sub sample names with TX and FL regexes + newnamesTX = re.sub(TXnames,"Cf.Sfa",Line) + newnamesALL = re.sub(FLnames,"Cf.Gai",newnamesTX) + #write new version of line to file + outfile.write(newnamesALL + "\n") + + else: + #replace full SNP info with allele counts only + AC=re.sub(ACR,r"\1",Line) + #replace missing data with NA + ACNA=re.sub(r"\.","NA",AC) + #write new version of line to new file + outfile.write(ACNA + "\n") + +#Close files +vcffile.close() +outfile.close() + diff --git a/Question2.py b/Question2.py new file mode 100755 index 0000000..50e9029 --- /dev/null +++ b/Question2.py @@ -0,0 +1,18 @@ +import pandas +import numpy +import re + +sequenceID = open("indivIDs.txt", "r") #Open sequence ID file + +barcodes={} #Define empty dictionary for barcodes associated with sequence IDs + +for line in sequenceID: #Loop through sequence ID file line-by-line + line=line.strip() #Strip whitespace from beginning and end of line + cols=line.split() #Return the first value and the second value as unique objects in a list + if cols[0] in barcodes: #Check if the first value in the line (the barcode) is already in the dictionary + print "Duplicate:" + cols[1] #Print error message + else: + barcodes[cols[0]] = cols[1] #If barcode is not yet present, add it as a key, and associate it with + #the second value in the line (the sequence ID) + +file.close(sequenceID) #Close the file