issues when running the step5.screen_high-confidence_intermolecular

[ZHIDIHUAYUAN]

Hello,
Thanks for the code. But when I run the code of recalibrate_pvalue part, I got a mistake. Illegal division by zero at /public/data1/zhoumy/RIC/code/step5.screen_high-confidence_intermolecular/scripts/5.recalibrate_pvalue/2.multiple_testing_correction.pl line 244, line 1281814. The data is processed as described in your article. Can I delete this line? Or how should I handle this situation? Run the simulation again?

Thank you.

[caochch]

Hi, The possible reason may be the sparsity of the input network. Increase sequencing depth or use larger window parameters (try 1000, 2000, instead of default 500). Best regards ZHIDIHUAYUAN ***@***.***> 于2024年4月26日周五 10:38写道：

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Hello, Thanks for the code. But when I run the code of recalibrate_pvalue part, I got a mistake. Illegal division by zero at /public/data1/zhoumy/RIC/code/step5.screen_high-confidence_intermolecular/scripts/5.recalibrate_pvalue/ 2.multiple_testing_correction.pl line 244, line 1281814. The data is processed as described in your article. Can I delete this line? Or how should I handle this situation? Run the simulation again? Thank you. — Reply to this email directly, view it on GitHub <#8>, or unsubscribe <https://github.com/notifications/unsubscribe-auth/AIJXTAAIK6YLXOZV55SEZZDY7G433AVCNFSM6AAAAABGZ65L32VHI2DSMVQWIX3LMV43ASLTON2WKOZSGI3DIOBUG42DIMA> . You are receiving this because you are subscribed to this thread.Message ID: ***@***.***>

[ZHIDIHUAYUAN]

Thank you.
I have some problems in understanding the ChimericWhole alignment. In my output of Chimeric.sam, there is a read:
Head_SRR17136186.48579355 272 chr3 128768424 3 107S27M
Tail_SRR17136186.48579355 16 chr3 128768441 3 107M27S
there is an overlap between the two fragments.
How can I understand it? Should I discard the read?