ms stats shiny wont read annotation file for processing of fragpipe output

[johnkollias]

Hello!

I have run processing on fragpipe the data of a dia experiment. i want to run the output pg_matrix.tsv and the shiny crashes when i start and I get the message:

Warning: Error in .checkAnnotation: ** Columns Run, Condition, BioReplicate missing in the annotation. Please check the annotation file.
143: stop
142: .checkAnnotation
141: MSstatsConvert::MSstatsMakeAnnotation
140: FragPipetoMSstatsFormat
139: getData
138: eventReactiveValueFunc
86: get_data
85: eval [#2]
84: eval
83: observe
82: observer:observeEvent(session$input[[shinyInputId]])
3: runApp
2: print.shiny.appobj
1:

on Rstudio. I have tried both tsv and csv annotations and the column names are as requested (Run,Condition,BioReplicate)