I have not investigated the origins of this problem; maybe you could venture a guess and save me some time.
AssembleJSeq.py produces no output because, indeed, there is no overlap between the 5' and 3' read IDs. I do not know if that is a problem, but the empty file causes novoindex to crash down the line.
Here's how far I got (non-empty files are highlighted in green):
test.pdf
Stuck here:

I have not investigated the origins of this problem; maybe you could venture a guess and save me some time.
AssembleJSeq.pyproduces no output because, indeed, there is no overlap between the 5' and 3' read IDs. I do not know if that is a problem, but the empty file causesnovoindexto crash down the line.Here's how far I got (non-empty files are highlighted in green):
test.pdf
Stuck here: