MUSE compatibility with hg38 reference?

[W4RJ4]

Hi, I recently tried running MuSE with hg38 reference and appropriate hg38 dbsnp database. After 10 hours I got segmentation fault(core dumped) on chr 10. And what i want to ask here is, if I made a mistake by running it at all with hg38 or there is maybe another reason why error occurred on this region?

[buchanae]

@W4RJ4 I can't think of why MUSE would be specific to one reference. @kamichiotti are you running your analysis on hg38?

[kamichiotti]

@buchanae @W4RJ4 I have been using hg19, but there shouldn't be any reason it wouldn't work on hg38 provided the files are correctly formatted and compressed. The error may be due large input BAM, particularly WGS. In this case, implementing the -r (single region (chr:pos-pos) where somatic mutations are to be called; STRING) or -l (list of regions (chr:pos-pos or BED) where somatic mutations are to be called, one region per line; FILE) options.