how can l combine the result of augustus,RAKER1 and BRAKER2 result?

[ld9866]

Hello, I'm annotating the genome of an animal. The usual way is to merge the predicted results. You only have how to combine the files of braker1 and braker2 in the example, so if I have the results of Augustus and snap data, how can I combine all of them?
thanks!

[LarsGab]

Hi,

we moved the repository for TSEBRA to: https://github.com/Gaius-Augustus/TSEBRA
The repository here isn't used for further development and the above repo is more up to date.

You can add your Augustus gene set by adding your Augustus file(s) to the comma-separated list of gtf files.
For example, if your Augustus file is called augustus.gtf, the TSBERA command could look like:

./bin/tsebra.py -g braker1.gtf,braker2.gtf,augustus.gtf -c default.cfg \ 
    -e braker1_out/hintsfile.gff,braker2_out/hintsfile.gff \
    -o braker1+2_combined.gtf

I would recommend that you use the BRAKER hintsfiles as evidence. If you have additional evidence, you have to make sure that they are in the correct format (see BRAKER hints) and then you can add them to the extrinsic evidence list behind the -e option.
Best,
Lars