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_quarto.yml
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project:
type: website
output-dir: docs
render:
- "vignettes/*.qmd"
- "!vignettes/guides/lif-cachexia-validation-framework.qmd"
- "vignettes/under-construction.qmd"
- "vignettes/introduction/*.qmd"
- "vignettes/setup/*.qmd"
- "vignettes/gtex/*.qmd"
- "vignettes/kpc-cross-study/sra-fetch-prjna773714.qmd"
- "vignettes/kpc-cross-study/prjna773714-rna-qc.qmd"
- "vignettes/kpc-cross-study/st003927-metabolomics-qc.qmd"
- "vignettes/kpc-cross-study/prjna773714-rna-gsea.qmd"
- "vignettes/kpc-cross-study/st003927-metabolomics-msea.qmd"
- "vignettes/kpc-cross-study/gse133523-cachexia-gsea.qmd"
- "vignettes/kpc-cross-study/kpc-rna-metabolomics-cachexia.qmd"
- "vignettes/integration/*.qmd"
- "vignettes/guides/*.qmd"
- "vignettes/reference/**/*.qmd"
execute:
engine: knitr
cache: false
freeze: auto
website:
page-navigation: true
back-to-top-navigation: true
page-footer: ""
title: "Multi-Omics Analysis: From Public Data to Biological Insight"
navbar:
left:
- text: "Home"
href: vignettes/index.qmd
- text: "Workshop Flow"
menu:
- text: "Prerequisites"
- text: "Background & Biology"
href: vignettes/introduction/lif-cachexia-biology-background.qmd
- text: "Environment Setup"
href: vignettes/setup/env-setup.qmd
- text: "---"
- text: "Cross-Study Integration Demo"
- text: "SRA Data Fetch (PRJNA773714)"
href: vignettes/kpc-cross-study/sra-fetch-prjna773714.qmd
- text: "RNA-seq QC"
href: vignettes/kpc-cross-study/prjna773714-rna-qc.qmd
- text: "Metabolomics QC"
href: vignettes/kpc-cross-study/st003927-metabolomics-qc.qmd
- text: "RNA-seq GSEA"
href: vignettes/kpc-cross-study/prjna773714-rna-gsea.qmd
- text: "Metabolomics MSEA"
href: vignettes/kpc-cross-study/st003927-metabolomics-msea.qmd
- text: "8. Human Cachexia GSEA (GSE133523)"
href: vignettes/kpc-cross-study/gse133523-cachexia-gsea.qmd
- text: "9. KPC RNA–Metabolomics Concordance"
href: vignettes/kpc-cross-study/kpc-rna-metabolomics-cachexia.qmd
- text: "10. GTEx–Cachexia NES Concordance"
href: vignettes/integration/gtex-cachexia-nes-concordance.qmd
- text: "11. Conclusion: LIF Biology and Cachexia"
href: vignettes/integration/kpc-lif-cachexia-concordance.qmd
- text: "---"
- text: "GTEx Workshop"
- text: "1. GTEx Sample Metadata Access"
href: vignettes/gtex/gtex-meta-data-access.qmd
- text: "2. GTEx Expression Data Download (Portal .gct)"
href: vignettes/gtex/gtex-expression-access.qmd
- text: "3. Sample Quality Assessment"
href: vignettes/gtex/gtex-sample-qa.qmd
- text: "4. Expression Data Structure"
href: vignettes/gtex/gtex-expression-cache-structure.qmd
- text: "5. Expression Prep & QC"
href: vignettes/gtex/gtex-expression-prep-qc.qmd
- text: "6. Expression Analysis"
href: vignettes/gtex/gtex-expression-lif-analysis.qmd
- text: "---"
- text: "Interpretation & Translation"
- text: "Spearman Correlation Deep Dive"
href: vignettes/guides/lif-spearman-correlation-guide.qmd
- text: "GSEA & GO Enrichment"
href: vignettes/guides/go-enrichment-gsea-guide.qmd
- text: "Cachexia Validation Framework"
href: vignettes/under-construction.qmd
- text: "References"
menu:
- text: "Acronym & Term Index"
href: vignettes/guides/acronym-index.qmd
- text: "---"
- text: "R: Workshop Function References"
href: vignettes/reference/helpers/workshopr.qmd
- text: "Global References"
href: vignettes/reference/references.qmd
format:
html:
theme:
light: [cosmo, vignettes/custom.scss]
dark: [darkly, vignettes/custom-dark.scss]
page-layout: full
toc: true
toc-location: left
number-sections: false
search: true
code-overflow: scroll
image-lazy-loading: true
code-tools: true
code-copy: true
fig-cap-location: bottom
citations-hover: true
include-in-header:
text: |
<link rel="preconnect" href="https://fonts.googleapis.com">
<link rel="preconnect" href="https://fonts.gstatic.com" crossorigin>
<link rel="stylesheet" href="https://fonts.googleapis.com/css2?family=Atkinson+Hyperlegible:wght@400;700&family=JetBrains+Mono:wght@400;500;600&display=swap">