diff --git a/serverless/src/lambda/layer-catalog.ts b/serverless/src/lambda/layer-catalog.ts
index a925aad..456019b 100644
--- a/serverless/src/lambda/layer-catalog.ts
+++ b/serverless/src/lambda/layer-catalog.ts
@@ -54,10 +54,6 @@ export const lambdaLayerCatalog = {
       runtimeType: "JAVA",
       tracerLayerNames: { x86_64: "dd-trace-java", arm64: "dd-trace-java" },
     },
-    "nodejs12.x": {
-      runtimeType: "NODE",
-      tracerLayerNames: { x86_64: "Datadog-Node12-x", arm64: "Datadog-Node12-x" },
-    },
     "nodejs14.x": {
       runtimeType: "NODE",
       tracerLayerNames: { x86_64: "Datadog-Node14-x", arm64: "Datadog-Node14-x" },
@@ -90,14 +86,6 @@ export const lambdaLayerCatalog = {
       runtimeType: "CUSTOM",
       tracerLayerNames: { x86_64: null, arm64: null },
     },
-    "python2.7": {
-      runtimeType: "PYTHON",
-      tracerLayerNames: { x86_64: "Datadog-Python27", arm64: null },
-    },
-    "python3.6": {
-      runtimeType: "PYTHON",
-      tracerLayerNames: { x86_64: "Datadog-Python36", arm64: null },
-    },
     "python3.7": {
       runtimeType: "PYTHON",
       tracerLayerNames: { x86_64: "Datadog-Python37", arm64: null },
diff --git a/serverless/test/index.spec.ts b/serverless/test/index.spec.ts
index 4def0e4..027921f 100644
--- a/serverless/test/index.spec.ts
+++ b/serverless/test/index.spec.ts
@@ -47,7 +47,7 @@ export function mockInputEvent(
           Role: {
             "Fn::GetAtt": ["HelloWorldFunctionRole", "Arn"],
           },
-          Runtime: "nodejs12.x",
+          Runtime: "nodejs24.x",
         },
       },
     },
diff --git a/serverless/test/lambda/env.spec.ts b/serverless/test/lambda/env.spec.ts
index 0a30064..5e33cc0 100644
--- a/serverless/test/lambda/env.spec.ts
+++ b/serverless/test/lambda/env.spec.ts
@@ -162,13 +162,13 @@ describe("setEnvConfiguration", () => {
     const lambda: LambdaFunction = {
       properties: {
         Handler: "app.handler",
-        Runtime: "python2.7",
+        Runtime: "python3.14",
         Role: "role-arn",
         Code: {},
       },
       key: "FunctionKey",
       runtimeType: RuntimeType.PYTHON,
-      runtime: "python2.7",
+      runtime: "python3.14",
       architecture: "x86_64",
       architectureType: ArchitectureType.x86_64,
     };
@@ -204,13 +204,13 @@ describe("setEnvConfiguration", () => {
     const lambda: LambdaFunction = {
       properties: {
         Handler: "app.handler",
-        Runtime: "python2.7",
+        Runtime: "python3.14",
         Role: "role-arn",
         Code: {},
       },
       key: "FunctionKey",
       runtimeType: RuntimeType.PYTHON,
-      runtime: "python2.7",
+      runtime: "python3.14",
       architecture: "x86_64",
       architectureType: ArchitectureType.x86_64,
     };
@@ -244,13 +244,13 @@ describe("setEnvConfiguration", () => {
     const lambda: LambdaFunction = {
       properties: {
         Handler: "app.handler",
-        Runtime: "python2.7",
+        Runtime: "python3.14",
         Role: "role-arn",
         Code: {},
       },
       key: "FunctionKey",
       runtimeType: RuntimeType.PYTHON,
-      runtime: "python2.7",
+      runtime: "python3.14",
       architecture: "x86_64",
       architectureType: ArchitectureType.x86_64,
     };
@@ -318,13 +318,13 @@ describe("setEnvConfiguration", () => {
     const lambda: LambdaFunction = {
       properties: {
         Handler: "app.handler",
-        Runtime: "python2.7",
+        Runtime: "python3.14",
         Role: "role-arn",
         Code: {},
       },
       key: "FunctionKey",
       runtimeType: RuntimeType.PYTHON,
-      runtime: "python2.7",
+      runtime: "python3.14",
       architecture: "x86_64",
       architectureType: ArchitectureType.x86_64,
     };
@@ -405,14 +405,14 @@ describe("setEnvConfiguration", () => {
     const lambda: LambdaFunction = {
       properties: {
         Handler: "app.handler",
-        Runtime: "python2.7",
+        Runtime: "python3.14",
         Role: "role-arn",
         Code: {},
         Environment: { Variables: originalEnvVars },
       },
       key: "FunctionKey",
       runtimeType: RuntimeType.PYTHON,
-      runtime: "python2.7",
+      runtime: "python3.14",
       architecture: "x86_64",
       architectureType: ArchitectureType.x86_64,
     };
@@ -453,13 +453,13 @@ describe("setEnvConfiguration", () => {
     const lambda: LambdaFunction = {
       properties: {
         Handler: "app.handler",
-        Runtime: "python2.7",
+        Runtime: "python3.14",
         Role: "role-arn",
         Code: {},
       },
       key: "FunctionKey",
       runtimeType: RuntimeType.PYTHON,
-      runtime: "python2.7",
+      runtime: "python3.14",
       architecture: "x86_64",
       architectureType: ArchitectureType.x86_64,
     };
@@ -497,13 +497,13 @@ describe("setEnvConfiguration", () => {
     const lambda: LambdaFunction = {
       properties: {
         Handler: "app.handler",
-        Runtime: "python2.7",
+        Runtime: "python3.14",
         Role: "role-arn",
         Code: {},
       },
       key: "FunctionKey",
       runtimeType: RuntimeType.PYTHON,
-      runtime: "python2.7",
+      runtime: "python3.14",
       architecture: "x86_64",
       architectureType: ArchitectureType.x86_64,
     };
@@ -542,13 +542,13 @@ describe("setEnvConfiguration", () => {
     const lambda: LambdaFunction = {
       properties: {
         Handler: "app.handler",
-        Runtime: "python2.7",
+        Runtime: "python3.14",
         Role: "role-arn",
         Code: {},
       },
       key: "FunctionKey",
       runtimeType: RuntimeType.PYTHON,
-      runtime: "python2.7",
+      runtime: "python3.14",
       architecture: "x86_64",
       architectureType: ArchitectureType.x86_64,
     };
@@ -582,13 +582,13 @@ describe("setEnvConfiguration", () => {
     const lambda: LambdaFunction = {
       properties: {
         Handler: "app.handler",
-        Runtime: "python2.7",
+        Runtime: "python3.14",
         Role: "role-arn",
         Code: {},
       },
       key: "FunctionKey",
       runtimeType: RuntimeType.PYTHON,
-      runtime: "python2.7",
+      runtime: "python3.14",
       architecture: "x86_64",
       architectureType: ArchitectureType.x86_64,
     };
@@ -622,13 +622,13 @@ describe("setEnvConfiguration", () => {
     const lambda: LambdaFunction = {
       properties: {
         Handler: "app.handler",
-        Runtime: "python2.7",
+        Runtime: "python3.14",
         Role: "role-arn",
         Code: {},
       },
       key: "FunctionKey",
       runtimeType: RuntimeType.PYTHON,
-      runtime: "python2.7",
+      runtime: "python3.14",
       architecture: "x86_64",
       architectureType: ArchitectureType.x86_64,
     };
@@ -695,13 +695,13 @@ describe("setEnvConfiguration", () => {
       properties: {
         FunctionName: "my-function",
         Handler: "app.handler",
-        Runtime: "nodejs12.x",
+        Runtime: "nodejs24.x",
         Role: "role-arn",
         Code: {},
       },
       key: "FunctionKey",
       runtimeType: RuntimeType.NODE,
-      runtime: "nodejs12.x",
+      runtime: "nodejs24.x",
       architecture: "x86_64",
       architectureType: ArchitectureType.x86_64,
     };
@@ -776,13 +776,13 @@ describe("setEnvConfiguration", () => {
       properties: {
         FunctionName: "my-function",
         Handler: "app.handler",
-        Runtime: "nodejs12.x",
+        Runtime: "nodejs24.x",
         Role: "role-arn",
         Code: {},
       },
       key: "FunctionKey",
       runtimeType: RuntimeType.NODE,
-      runtime: "nodejs12.x",
+      runtime: "nodejs24.x",
       architecture: "x86_64",
       architectureType: ArchitectureType.x86_64,
     };
diff --git a/serverless/test/lambda/lambda.spec.ts b/serverless/test/lambda/lambda.spec.ts
index 8f709cb..c4604f6 100644
--- a/serverless/test/lambda/lambda.spec.ts
+++ b/serverless/test/lambda/lambda.spec.ts
@@ -43,9 +43,9 @@ describe("Lambda", () => {
       const output = await handler(inputEvent, {});
       const lambdaProperties: FunctionProperties = output.fragment.Resources[LAMBDA_KEY].Properties;
 
-      // Mocked Lambda has runtime nodejs12.x, so layer name is Datadog-Node12-x, with provided version number (25) at end
+      // Mocked Lambda has runtime nodejs24.x, so layer name is Datadog-Node24-x, with provided version number (25) at end
       expect(lambdaProperties.Layers).toEqual([
-        expect.stringMatching(/arn:aws:lambda:us-east-1:.*:layer:Datadog-Node12-x:25/),
+        expect.stringMatching(/arn:aws:lambda:us-east-1:.*:layer:Datadog-Node24-x:25/),
       ]);
     });
 
@@ -98,7 +98,7 @@ describe("Lambda", () => {
       const lambdaProperties: FunctionProperties = output.fragment.Resources[LAMBDA_KEY].Properties;
 
       expect(lambdaProperties.Layers).toEqual([
-        expect.stringMatching(/arn:aws-us-gov:lambda:us-gov-east-1:002406178527:layer:Datadog-Node12-x:25/),
+        expect.stringMatching(/arn:aws-us-gov:lambda:us-gov-east-1:002406178527:layer:Datadog-Node24-x:25/),
       ]);
     });
 
diff --git a/serverless/test/lambda/layer.spec.ts b/serverless/test/lambda/layer.spec.ts
index e9d200e..1f5bb33 100644
--- a/serverless/test/lambda/layer.spec.ts
+++ b/serverless/test/lambda/layer.spec.ts
@@ -61,7 +61,6 @@ describe("findLambdas", () => {
       Java25Function: mockFunctionResource("java25", ["x86_64"]),
       Java8Function: mockFunctionResource("java8", ["x86_64"]),
       Java8al2Function: mockFunctionResource("java8.al2", ["x86_64"]),
-      Node12Function: mockFunctionResource("nodejs12.x", ["x86_64"]),
       Node14Function: mockFunctionResource("nodejs14.x", ["x86_64"]),
       Node16Function: mockFunctionResource("nodejs16.x", ["x86_64"]),
       Node18Function: mockFunctionResource("nodejs18.x", ["x86_64"]),
@@ -70,8 +69,6 @@ describe("findLambdas", () => {
       Node24Function: mockFunctionResource("nodejs24.x", ["x86_64"]),
       ProvidedAl2Function: mockFunctionResource("provided.al2", ["x86_64"]),
       ProvidedAl2023Function: mockFunctionResource("provided.al2023", ["x86_64"]),
-      Python27Function: mockFunctionResource("python2.7", ["x86_64"]),
-      Python36Function: mockFunctionResource("python3.6", ["x86_64"]),
       Python37Function: mockFunctionResource("python3.7", ["x86_64"]),
       Python38Function: mockFunctionResource("python3.8", ["x86_64"]),
       Python39Function: mockFunctionResource("python3.9", ["x86_64"]),
@@ -99,7 +96,6 @@ describe("findLambdas", () => {
       mockLambdaFunction("Java25Function", "java25", RuntimeType.JAVA, "x86_64", ArchitectureType.x86_64),
       mockLambdaFunction("Java8Function", "java8", RuntimeType.JAVA, "x86_64", ArchitectureType.x86_64),
       mockLambdaFunction("Java8al2Function", "java8.al2", RuntimeType.JAVA, "x86_64", ArchitectureType.x86_64),
-      mockLambdaFunction("Node12Function", "nodejs12.x", RuntimeType.NODE, "x86_64", ArchitectureType.x86_64),
       mockLambdaFunction("Node14Function", "nodejs14.x", RuntimeType.NODE, "x86_64", ArchitectureType.x86_64),
       mockLambdaFunction("Node16Function", "nodejs16.x", RuntimeType.NODE, "x86_64", ArchitectureType.x86_64),
       mockLambdaFunction("Node18Function", "nodejs18.x", RuntimeType.NODE, "x86_64", ArchitectureType.x86_64),
@@ -114,8 +110,6 @@ describe("findLambdas", () => {
         "x86_64",
         ArchitectureType.x86_64,
       ),
-      mockLambdaFunction("Python27Function", "python2.7", RuntimeType.PYTHON, "x86_64", ArchitectureType.x86_64),
-      mockLambdaFunction("Python36Function", "python3.6", RuntimeType.PYTHON, "x86_64", ArchitectureType.x86_64),
       mockLambdaFunction("Python37Function", "python3.7", RuntimeType.PYTHON, "x86_64", ArchitectureType.x86_64),
       mockLambdaFunction("Python38Function", "python3.8", RuntimeType.PYTHON, "x86_64", ArchitectureType.x86_64),
       mockLambdaFunction("Python39Function", "python3.9", RuntimeType.PYTHON, "x86_64", ArchitectureType.x86_64),
@@ -149,19 +143,19 @@ describe("findLambdas", () => {
 
 describe("applyLayers", () => {
   it("adds a layer array if none are present", () => {
-    const lambda = mockLambdaFunction("FunctionKey", "nodejs12.x", RuntimeType.NODE, "x86_64");
+    const lambda = mockLambdaFunction("FunctionKey", "nodejs24.x", RuntimeType.NODE, "x86_64");
     const region = "us-east-1";
     const nodeLayerVersion = 25;
     const errors = applyLayers(region, [lambda], undefined, nodeLayerVersion);
 
     expect(errors.length).toEqual(0);
     expect(lambda.properties.Layers).toEqual([
-      `arn:aws:lambda:${region}:${DD_ACCOUNT_ID}:layer:Datadog-Node12-x:${nodeLayerVersion}`,
+      `arn:aws:lambda:${region}:${DD_ACCOUNT_ID}:layer:Datadog-Node24-x:${nodeLayerVersion}`,
     ]);
   });
 
   it("appends to the layer array if already present", () => {
-    const lambda = mockLambdaFunction("FunctionKey", "nodejs12.x", RuntimeType.NODE, "x86_64");
+    const lambda = mockLambdaFunction("FunctionKey", "nodejs24.x", RuntimeType.NODE, "x86_64");
     lambda.properties.Layers = ["node:2"];
 
     const region = "us-east-1";
@@ -171,15 +165,15 @@ describe("applyLayers", () => {
     expect(errors.length).toEqual(0);
     expect(lambda.properties.Layers).toEqual([
       "node:2",
-      `arn:aws:lambda:${region}:${DD_ACCOUNT_ID}:layer:Datadog-Node12-x:${nodeLayerVersion}`,
+      `arn:aws:lambda:${region}:${DD_ACCOUNT_ID}:layer:Datadog-Node24-x:${nodeLayerVersion}`,
     ]);
   });
 
   it("doesn't add duplicate layers", () => {
-    const lambda = mockLambdaFunction("FunctionKey", "nodejs12.x", RuntimeType.NODE, "x86_64");
+    const lambda = mockLambdaFunction("FunctionKey", "nodejs24.x", RuntimeType.NODE, "x86_64");
     const region = "us-east-1";
     const nodeLayerVersion = 25;
-    const layerArn = `arn:aws:lambda:${region}:${DD_ACCOUNT_ID}:layer:Datadog-Node12-x:${nodeLayerVersion}`;
+    const layerArn = `arn:aws:lambda:${region}:${DD_ACCOUNT_ID}:layer:Datadog-Node24-x:${nodeLayerVersion}`;
     lambda.properties.Layers = [layerArn];
     const errors = applyLayers(region, [lambda], undefined, nodeLayerVersion);
 
@@ -196,8 +190,8 @@ describe("applyLayers", () => {
   });
 
   it("returns errors if layer versions are not provided for corresponding Lambda runtimes", () => {
-    const pythonLambda = mockLambdaFunction("PythonFunctionKey", "python2.7", RuntimeType.PYTHON, "x86_64");
-    const nodeLambda = mockLambdaFunction("NodeFunctionKey", "nodejs12.x", RuntimeType.NODE, "x86_64");
+    const pythonLambda = mockLambdaFunction("PythonFunctionKey", "python3.14", RuntimeType.PYTHON, "x86_64");
+    const nodeLambda = mockLambdaFunction("NodeFunctionKey", "nodejs24.x", RuntimeType.NODE, "x86_64");
     const errors = applyLayers("us-east-1", [pythonLambda, nodeLambda]);
 
     expect(errors).toEqual([
@@ -446,7 +440,7 @@ describe("getNewLayers", () => {
 describe("isGovCloud", () => {
   it("applies the GovCloud layer", () => {
     const pythonLambda = mockLambdaFunction("PythonFunctionKey", "python3.8", RuntimeType.PYTHON, "x86_64");
-    const nodeLambda = mockLambdaFunction("NodeFunctionKey", "nodejs16.x", RuntimeType.NODE, "x86_64");
+    const nodeLambda = mockLambdaFunction("NodeFunctionKey", "nodejs24.x", RuntimeType.NODE, "x86_64");
     const errors = applyLayers("us-gov-east-1", [pythonLambda, nodeLambda], 21, 30);
 
     expect(errors.length).toEqual(0);
@@ -454,25 +448,25 @@ describe("isGovCloud", () => {
       `arn:aws-us-gov:lambda:us-gov-east-1:${DD_GOV_ACCOUNT_ID}:layer:Datadog-Python38:21`,
     ]);
     expect(nodeLambda.properties.Layers).toEqual([
-      `arn:aws-us-gov:lambda:us-gov-east-1:${DD_GOV_ACCOUNT_ID}:layer:Datadog-Node16-x:30`,
+      `arn:aws-us-gov:lambda:us-gov-east-1:${DD_GOV_ACCOUNT_ID}:layer:Datadog-Node24-x:30`,
     ]);
   });
 });
 
 describe("getLambdaLibraryLayerArn", () => {
-  it("gets the us-east-1 layer arn for the Datadog Node16 Lambda Library", () => {
+  it("gets the us-east-1 layer arn for the Datadog Node24 Lambda Library", () => {
     const region = "us-east-1";
     const version = 22;
-    const runtime = "nodejs16.x";
+    const runtime = "nodejs24.x";
     const layerArn = getLambdaLibraryLayerArn(region, version, runtime, "x86_64");
-    expect(layerArn).toEqual(`arn:aws:lambda:${region}:${DD_ACCOUNT_ID}:layer:Datadog-Node16-x:${version}`);
+    expect(layerArn).toEqual(`arn:aws:lambda:${region}:${DD_ACCOUNT_ID}:layer:Datadog-Node24-x:${version}`);
   });
-  it("gets the us-east-1 layer arn for the Datadog Python36 Lambda Library", () => {
+  it("gets the us-east-1 layer arn for the Datadog Python314 Lambda Library", () => {
     const region = "us-east-1";
     const version = 22;
-    const runtime = "python3.6";
+    const runtime = "python3.14";
     const layerArn = getLambdaLibraryLayerArn(region, version, runtime, "x86_64");
-    expect(layerArn).toEqual(`arn:aws:lambda:${region}:${DD_ACCOUNT_ID}:layer:Datadog-Python36:${version}`);
+    expect(layerArn).toEqual(`arn:aws:lambda:${region}:${DD_ACCOUNT_ID}:layer:Datadog-Python314:${version}`);
   });
   it("gets the us-east-1 ARM layer arn for the Datadog Python38 Lambda Library", () => {
     const region = "us-east-1";
@@ -481,12 +475,12 @@ describe("getLambdaLibraryLayerArn", () => {
     const layerArn = getLambdaLibraryLayerArn(region, version, runtime, "arm64");
     expect(layerArn).toEqual(`arn:aws:lambda:${region}:${DD_ACCOUNT_ID}:layer:Datadog-Python38-ARM:${version}`);
   });
-  it("gets the us-gov-east-1 layer arn for the Datadog Python36 Lambda Library", () => {
+  it("gets the us-gov-east-1 layer arn for the Datadog Python38 Lambda Library", () => {
     const region = "us-gov-east-1";
     const version = 22;
-    const runtime = "python3.6";
+    const runtime = "python3.8";
     const layerArn = getLambdaLibraryLayerArn(region, version, runtime, "x86_64");
-    expect(layerArn).toEqual(`arn:aws-us-gov:lambda:${region}:${DD_GOV_ACCOUNT_ID}:layer:Datadog-Python36:${version}`);
+    expect(layerArn).toEqual(`arn:aws-us-gov:lambda:${region}:${DD_GOV_ACCOUNT_ID}:layer:Datadog-Python38:${version}`);
   });
   it("gets the us-gov-east-1 ARM layer arn for the Datadog Python39 Lambda Library", () => {
     const region = "us-gov-east-1";